Comparative analysis of gel image using PDQuest and Progenesis SameSpot. Two different experimental groups were analyzed. As shown in A and B, a protein spot of F-cappig protein was recognized as a multiple spots by PDQuest when spot detection approach was used. C shows a precise detection of the same protein spot when images were aligned first (Progenesis SameSpot). The detected spot was confirmed later on a 2D (D) and 3D (E) views to be an actual fragmented peptide ions.