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Figure 2 | Clinical Proteomics

Figure 2

From: Disrupting KATP channels diminishes the estrogen-mediated protection in female mutant mice during ischemia-reperfusion

Figure 2

Ischemia-reperfusion (I-R) response in SUR2KO and WT mice. (A) Treatment protocol. Grey arrows indicate endpoints when mouse hearts were harvested. (B) Survival% from KO and WT mice post IR. (C) Average infarct sizes recorded from IR-treated hearts. Infarcts are calculated as a ratio of infarcted area (INF) over area-at-risk (AAR). n = 5-7. *: p < 0.05 KO vs WT. AAR/LV ratios are shown in the supplement Additional file 1: Figure S1. (D) Quantitative RT-PCR of SUR2 transcripts in untreated (Con) and I-R hearts of WT mice. The signals were normalized to GAPDH. (E) Representative Western blots in mouse LV samples. Anti-SUR2 (BNJ2) was used as the primary antibody (1:1000). Secondary antibody was added at 1:10,000. The blots were stripped and hybridized with GAPDH (1:2000), and the density values were normalized to GAPDH (in arbitrary unit). In D- E, n = 4, *: p < 0.05.

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