Table 3 Identification of N-linked glycosylation sites using PNGase F with the incorporation of 18 O water in human adipocytokines
From: Quantitative secretome and glycome of primary human adipocytes during insulin resistance
No. | Protein ID | Identifed proteins | N-linked peptidesa |
|---|---|---|---|
1 | P15144 | Aminopeptidase N | KLN@YTLSQGHR |
2 | P21810 | Biglycan | LLQVVYLHSNN@ITK |
3 | P16870 | Carboxypeptidase E | GN@ETIVNLIHSTR |
4 | P07339 | Cathepsin D | GSLSYLN@VTR |
5 | P43235 | Cathepsin K | SN@DTLYIPEWEGR |
6 | P07711 | Cathepsin L | YSVAN@DTGFVDIPK |
7 | P07711 | Cathepsin L | YSVAN@DTGFVDIPKQEK |
8 | P10909 | Clusterin | LAN@LTQGEDQYYLR |
9 | P02461 | Collagen alpha-1(III) chain | ASQN@ITYHCK |
10 | P02461 | Collagen alpha-1(III) chain | DGSPGGKGDRGEN@GSPGAPGAPGHPGPPGPVGPAGK |
11 | P20908 | Collagen alpha-1(V) chain | VYCN@FTAGGSTCVFPDKK |
12 | P12109 | Collagen alpha-1(VI) chain | ENYAELLEDAFLKN@VTAQICIDKK |
13 | P12109 | Collagen alpha-1(VI) chain | GEDGPAGN@GTEGFPGFPGYPGNR |
14 | P12109 | Collagen alpha-1(VI) chain | N@FTAADWGQSR |
15 | P12109 | Collagen alpha-1(VI) chain | RN@FTAADWGQSR |
16 | Q02388 | Collagen alpha-1(VII) chain | TAPEPVGRVSRLQILN@ASSDVLR |
17 | Q99715 | Collagen alpha-1(XII) chain | EAGN@ITTDGYEILGK |
18 | P08123 | Collagen alpha-2(I) chain | LLANYASQN@ITYHCK |
19 | P05997 | Collagen alpha-2(V) chain | EASQN@ITYICK |
20 | P12111 | Collagen alpha-3(VI) chain | GNPGEPGLN@GTTGPKGIR |
21 | P12111 | Collagen alpha-3(VI) chain | GPPGVN@GTQGFQGCPGQR |
22 | P12111 | Collagen alpha-3(VI) chain | GYPGDEGGPGERGPPGVN@GTQGFQGCPGQR |
23 | P09871 | Complement C1s subcomponent | NCGVN@CSGDVFTALIGEIASPNYPKPYPENSR |
24 | O75462 | Cytokine receptor-like factor 1 | VLN@ASTLALALANLN@GSR |
25 | O75462 | Cytokine receptor-like factor 1 | VVDDVSN@QTSCR |
26 | P07585 | Decorin | IADTN@ITSIPQGLPPSLTELHLDGNK |
27 | P07585 | Decorin | LGLSFNSISAVDN@GSLANTPHLR |
28 | Q9UBP4 | Dickkopf-related protein 3 | GSN@GTICDNQR |
29 | Q13822 | Ectonucleotide pyrophosphatase/phosphodiesterase 2 | AEGWEEGPPTVLSDSPWTN@ISGSCK |
30 | Q13822 | Ectonucleotide pyrophosphatase/phosphodiesterase 2 | AIIAN@LTCK |
31 | Q9Y6C2 | EMILIN-1 | LGALN@SSLQLLEDR |
32 | P35555 | Fibrillin-1 | TAIFAFN@ISHVSNK |
33 | P02751 | Fibronectin | DQCIVDDITYNVN@DTFHKR |
34 | P02751 | Fibronectin | DQCIVDDITYNVN@DTFHK |
35 | P02751 | Fibronectin | LDAPTNLQFVN@ETDSTVLVR |
36 | Q12841 | Follistatin-related protein 1 | GSN@YSEILDK |
37 | Q12841 | Follistatin-related protein 1 | GSN@YSEILDKYFK |
38 | P09382 | Galectin-1 | FNAHGDANTIVCNSK |
39 | Q08380 | Galectin-3-binding protein | ALGFEN@ATQALGR |
40 | Q08380 | Galectin-3-binding protein | DAGVVCTN@ETR |
41 | Q08380 | Galectin-3-binding protein | TVIRPFYLTN@SSGVD |
42 | P00738 | Haptoglobin | VVLHPN@YSQVDIGLIK |
43 | O75629 | Human Protein CREG1 | LN@ITNIWVLDYFGGPK |
44 | P17936 | Insulin-like growth factor-binding protein 3 | GLCVN@ASAVSR |
45 | O14498 | ISLR | SLDLSHNLISDFAWSDLHN@LSALQLLK |
46 | Q8NHP8 | LAMA-like protein 2 | SDLNPAN@GSYPFKALR |
47 | Q16363 | Laminin subunit alpha-4 | DAVRN@LTEVVPQLLDQLR |
48 | Q16363 | Laminin subunit alpha-4 | FYFGGSPISAQYAN@FTGCISNAYFTR |
49 | Q16363 | Laminin subunit alpha-4 | LITEEAN@R |
50 | Q16363 | Laminin subunit alpha-4 | LTLSELDDIIKN@ASGIYAEIDGAK |
51 | Q16363 | Laminin subunit alpha-4 | RPASN@VSASIQR |
52 | P07942 | Laminin subunit beta-1 | LSDTTSQSN@STAK |
53 | P11047 | Laminin subunit gamma-1 | VN@NTLSSQISR |
54 | P11047 | Laminin subunit gamma-1 | KYEQAKN@ISQDLEK |
55 | P11047 | Laminin subunit gamma-1 | LLNN@LTSIK |
56 | P11047 | Laminin subunit gamma-1 | TAN@DTSTEAYNLLLR |
57 | P11047 | Laminin subunit gamma-1 | TLAGEN@QTAFEIEELNR |
58 | P11047 | Laminin subunit gamma-1 | VNDN@KTAAEEALR |
59 | Q14767 | Latent-transforming growth factor beta-binding protein 2 | DGTQQAVPLEHPSSPWGLN@LTEK |
60 | P51884 | Lumican | AFEN@VTDLQWLILDHNLLENSK |
61 | P51884 | Lumican | LGSFEGLVN@LTFIHLQHNR |
62 | P51884 | Lumican | LHINHNN@LTESVGPLPK |
63 | P13473 | Lysosome-associated membrane glycoprotein 2 | IAVQFGPGFSWIAN@FTK |
64 | P01033 | Metalloproteinase inhibitor 1 | AKFVGTPEVN@QTTLYQR |
65 | P01033 | Metalloproteinase inhibitor 1 | FVGTPEVN@QTTLYQR |
66 | P01033 | Metalloproteinase inhibitor 1 | SHN@RSEEFLIAGK |
67 | Q9NRN5 | Olfactomedin-like protein 3 | IYVLDGTQN@DTAFVFPR |
68 | P26022 | Pentraxin-related protein PTX3 | ATDVLN@K |
69 | Q15063 | Periostin | EVN@DTLLVNELK |
70 | Q15063 | Periostin | IFLKEVN@DTLLVNELK |
71 | P98160 | Perlecan | SLTQGSLIVGDLAPVN@GTSQGK |
72 | P55058 | Phospholipid transfer protein | VSN@VSCQASVSR |
73 | P36955 | Pigment epithelium-derived factor | VTQN@LTLIEESLTSEFIHDIDR |
74 | P36955 | Pigment epithelium-derived factor | VTQN@LTLIEESLTSEFIHDIDRELK |
75 | P05155 | Plasma protease C1 inhibitor | VGQLQLSHN@LSLVILVPQNLK |
76 | P05155 | Plasma protease C1 inhibitor | VLSN@NSDANLELINTWVAK |
77 | P07602 | Proactivator polypeptide [Contains: Saposin-A] | TN@STFVQALVEHVK |
78 | P07602 | Proactivator polypeptide [Contains: Saposin-A] | LIDNN@KTEK |
79 | P07602 | Proactivator polypeptide [Contains: Saposin-A] | LIDNN@KTEKEILDAFDK |
80 | P07602 | Proactivator polypeptide [Contains: Saposin-A] | NLEKN@STK |
81 | P07602 | Proactivator polypeptide [Contains: Saposin-A] | NLEKN@STKQEILAALEK |
82 | P07602 | Proactivator polypeptide [Contains: Saposin-A] | TN@STFVQALVEHVKEECDR |
83 | P09486 | SPARC | VCSNDN@K |
84 | P09486 | SPARC | VCSNDN@KTFDSSCHFFATK |
85 | P24821 | Tenascin | N@TTSYVLR |
86 | P24821 | Tenascin | LN@YSLPTGQWVGVQLPR |
87 | P07996 | Thrombospondin-1 | VVN@STTGPGEHLR |
88 | P35442 | Thrombospondin-2 | VVN@STTGTGEHLR |
89 | Q6FGX5 | TIMP1 protein | FVGTPEVN@QTTLYQR |
90 | Q6FGX5 | TIMP1 protein | SHN@RSEEFLIAGK |
91 | Q6EMK4 | Vasorin | LHEITN@ETFR |