Figure 1

Diagram of workflow to determine altered histones PTMs in colon carcinoma tissues. Histones were isolated from whole tissue sections by acidic extraction using Shechter et al. protocol [36] followed by separation using SDS-PAGE and silver staining. The gel part containing histone core proteins were excised next proteins were digested with trypsin and subjected to MS analyses. MS/MS runs of pooled samples were performed to identify in deep the peptides that compose the collection of samples. The custom peptide database was further overlaid on individual 2D maps acquired in LC-MS runs. Maps were then used as the basis to quantify and point modified peptides with differential abundance between CRC and normal mucosa samples.