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Figure 3 | Clinical Proteomics

Figure 3

From: Histone H3 lysine 27 acetylation is altered in colon cancer

Figure 3

H3K27Ac is upregulated in colon carcinoma tissues. (A) Results of H3 and H3K27Ac immunostaining in 12 pairs of CRC tissues and normal mucosa and graphical depiction of densitometric assessment of differences (B). Histones were isolated from whole tissue sections by acidic extraction and equal amounts of protein were separated by SDS-PAGE, electrotransferred to PVDF membranes and immunostained with H3 (ab1791) or H3K27Ac (ab4729) antibody. Densitometric measurements were performed using OptiQuant image analysis software. H3K27Ac level was normalised to the signal from total H3. C, CRC- colon cancer; N, NC- normal colonic mucosa.

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