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Figure 2 | Clinical Proteomics

Figure 2

From: Differential analysis of N-glycoproteome between hepatocellular carcinoma and normal human liver tissues by combination of multiple protease digestion and solid phase based labeling

Figure 2

The venn diagram showing the overlap of the quantified N-glycosites by using three different digestion strategies (trypsin, trypsin & Glu-C, chymotrypsin). (A) evaluation experiment, (B) differential analysis experiment. For the evaluation experiment, two aliquots of the same protein extract from normal human liver tissue were light and heavy labeled to evaluate the performance of the integrated workflow. For the differential analysis experiment, the samples of normal and HCC human liver tissues were labeled with light and heavy dimethyl labels, respectively. One and three replicate 2D nanoLC-MS/MS runs of the labeled sample were carried out for the evaluation experiment and differential analysis experiment, respectively.

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