Figure 4From: Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples: insights from liver tissueQuantitative protein distribution according to physicochemical features. Quantitative protein distribution according to MW (A), pI (B), number of transmembrane domains (TMD, C) and hydrophobicity (GRAVY score, D). Mean and SD value of NSAF percentage for three independent experimental replicates are shown. NSAF values were expressed as percentage of all proteins. Asterisks indicate statistical significance according to Student’s t-test (p value < 0.05); the blue ones indicate statistically significant difference versus DT, the red ones versus FASP, the green ones versus ISD and the black ones versus all other methods, respectively.Back to article page