Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients

Table 1 Precursor-fragment transitions significantly different between OA and control patients

Protein name	Precursor sequence	Q1(charge)^a	Q3(ion)^b	XIC^c	DIGE^d,e		SRM^e
Protein name	Precursor sequence	Q1(charge)^a	Q3(ion)^b	XIC^c	Ratio p-value		Ratio p-value
Tubulin α6	DVNAAIATIK	508.3(+2)	801.4(y₈)	a	−1,76	0.007	−2.17	0.032
Tubulin α6	LISQIVSSITASLR	744.4(+2)	933.5(y₉)	b	−1,76	0.007	−2.17	0.017
Cathepsin B chain B	LPASFDAR	438.7(+2)	666.3(y₆)	c	+1.90	0.036	+4.21	0.005
Cathepsin B chain B	NGPVEGAFSVYSDFLLYK	1003.5(+2)	1048.5(y₈)	d	+1.90	0.036	+4.21	0.031
Dihydropyrimidinase-like 2 variant	VFNLYPR	454.7(+2)	662.3(y₅)	e	−2.52	0.028	−4.25	0.006
	IAVGSDADLVIWDPDSVK	950.5(+2)	545.7(y₆)	f				0.033
	IAVGSDADLVIWDPDSVK	950.5(+2)	846.4(y₇)	g				0.008
L-caldesmon II	GNVFSSPTAAGTPNK	724.4(+2)	856.5(y₉)	h	−1,93	0.008	−2.89	0.009
Destrin isoform B	YALYDASFETK	654.3(+2)	797.4(y₇)	i	+4.30	0.006	+4.02	0.025

^aPeptide m/z value filtered in the fisrt quadrupole; ^bFragment m/z value filtered in the third quadrupole; ^cAs depicted in Figure 1; ^dFrom the comparative analysis of differential protein expression [6]; ^eThe symbols ‘ + ’ and ‘-’ indicate increased or decreased abundance in OA samples relative to controls, respectively; DIGE, differential in-gel electrophoresis; m/z, mass-to-charge ratio; OA, osteoarthritis; SRM, selected reaction monitoring; XIC, extracted ion chromatogram.

ISSN: 1559-0275