Assessment of HER-2 expression status by well-based RPPA in human breast FFPE tissue specimens. (A) Cases with 3+ score had significantly higher HER-2 expression than those with 0/1+ (P < 0.001) and 2+ score (P = 0.001). However, there is no significant difference between 0/1+ and 2+ subgroups (P = 0.837). (B) Tumor specimens with FISH positive showed significantly high HER-2 expressional values compare with that of FISH negative (mean of 1.233 versus 0.407, P = 0.012) in IHC 2+ subgroup. Relative HER-2 expressional signals were calculated the ratio of HER-2/GAPDH and the value expressed as box plot.