HER-2 ROC curve and levels in breast cancer specimens. (A) ROC curve for well-based RPPA assay results in distinguishing between HER-2 postive and negative cases. (B) Individual HER-2 expression levels showed in 0/1+ (n = 32), 2+ (n = 15) and 3+ (n = 29) subgroups. Positive (●) and negative (○) were categorized according to the current ASCO/CAP guideline. A value of cutoff was 0.0689.