Figure 6

Analysis of purified plasma sample from healthy volunteer by ESI-MS. A: extracted ion chromatogram of the ion 784.4 ± 0.5 m/z corresponding to the native angiotensin II and a characteristic fragment spectrum with labeling of the b6-fragment (grey shaded area) B: extracted ion chromatogram of the ion 794.4 ± 0.5 m/z corresponding to the stable isotope labeled angiotensin II and a characteristic fragment spectrum with labeling of the b6-fragment (grey shaded area). Confirmation of the identity of the native ngiotensin II was performed by using both: retention time and fragment ion of co-eluting stable isotope ngiote ngiotensin II.