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Table 1 LC-gradient used for the separation of the plasma samples

From: Absolute quantification of endogenous angiotensin II levels in human plasma using ESI-LC-MS/MS

Time [min] Eluent A [%] Eluent B [%]
0 100 0
0.1 80 20
8 75 25
10 0 100
11 0 100
11.1 100 0
25 100 0