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Figure 1 | Clinical Proteomics

Figure 1

From: Assessment of the influence of the patient’s inflammatory state on the accuracy of a haptoglobin selected reaction monitoring assay

Figure 1

Calibration of the haptoglobin SRM assay. External calibration of the SRM assay was performed with the N Protein Standard SL (Siemens) used for the nephelometric assay calibration. Calibration solution was prepared according to the manufacturer’s instructions to a final concentration of 1150 mg/L. Dilutions were made in water at 575, 230, and 115 mg/L. A four points calibration was built ranging from 115 mg/l to 1150 mg/L. Each calibration point was injected in triplicate. A) Calibration curves obtained from three independent calibrations (different trypsin digestion) performed on the same day. B) Calibration curves obtained from two independent calibrations (different trypsin digestion) performed on different days.

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Clinical Proteomics

ISSN: 1559-0275