TRIS effect of on protein extraction efficacy from FFPE liver tissues probed by Western blots. FFPE liver was deparaffinized, and homogenized in 20, 100 and 500 mM TRIS–HCl, pH 8.0, respectively, all containing 2% SDS. Subsequently, proteins were extracted from tissue homogenate by incubating at 90°C for 0, 30, 60, 90 and 120 min, respectively. (A) Western blotting against Grp78, Ass and Ldha of proteins extracted from FFPE liver tissue. (B) The densitometric comparison of the band intensities in (A). The mean and SD values of band intensities for three independent experimental triplicates (n = 3) are shown.