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Table 1 Proteins differentially regulated in different fibroblasts upon inflammatory activation

From: Plasticity of fibroblasts demonstrated by tissue-specific and function-related proteome profiling

AccNr Protein name Skin fibroblasts NHLF   BM fibroblasts  
Supernatant:   Control IL-1β-treated Control IL-1β-treated Control IL-1β-treated
P05231 IL-6 _ +++ _ ++ _ +++
P10145 Il-8 _ +++ _ ++ _ ++
P09341 GRO-alpha/CXCl1 _ ++ _ +++ _ ++
P80162 CXCL6 _ ++ _ ++ _ ++
P42830 CXCL5 _ ++ _ ++ _ _
P08254 MMP-3/Stromelysin-1 _ +++ _ +++ _ _
P07585 Decorin ++ ++++ +++ ++++ ++ ++
P08253 MMP-2 ++ ++++ +++ +++ ++ +++
P09603 CSF-1 _ ++ ++ ++ _ ++
P98066 TSG-6 _ ++ _ _ _ ++
P26022 PTX3/ TSG-14 +++ +++ _ ++ +++ ++++
P19875 GRO-beta /CXCL2 _ ++ _ _ _ _
P05121 PAI-1 +++ +++ ++ +++ +++ +++
O00391 Sulfhydryl oxidase 1 (+) ++ ++++ +++ _ ++
Q15063 Periostin (+) _ ++ +++ (+) ++
Q9Y240 SCGF ++ _ (+) ++ _ _
Cytoplasm:        
O00469 PLOD-2 (+) ++ _ +++ (+) +++
P05362 ICAM-1 (CD54) _ _ (+) + _ ++
P05120 PAI-2 ++ +++ _ +++ ++ _
Nuclear extract:        
Q8WX93 Palladin ++ +++ ++ +++ ++ +++
P51911 Calponin-1 +++ +++ ++ +++ ++ +++
Q16666 γ-IFN-inducible protein 16 ++ ++ ++ ++ ++ +++
  1. The total number of distinct peptides identified per protein when considering all three sub-cellular fractions was used as a semi-quantitative measure for protein abundances in the different fibroblasts, which allowed determining up- or down-regulation of proteins upon inflammatory activation. Most of the regulated proteins were found in the secretomes, listed under “Supernatant”.
  2. AccNr, Swiss-Prot accession number; -, protein was not detected; +, protein was detected with one peptide; ++, protein was detected with 2 to 5 peptides; +++, protein was detected with ≥6 peptides; ++++, protein was detected with ≥15 peptides; (+), protein identification was ambiguous, i.e. protein was not found robustly in all corresponding samples.