Plasticity of fibroblasts demonstrated by tissue-specific and function-related proteome profiling

Table 1 Proteins differentially regulated in different fibroblasts upon inflammatory activation

AccNr	Protein name	Skin fibroblasts		NHLF		BM fibroblasts
Supernatant:		Control	IL-1β-treated	Control	IL-1β-treated	Control	IL-1β-treated
P05231	IL-6	_	+++	_	++	_	+++
P10145	Il-8	_	+++	_	++	_	++
P09341	GRO-alpha/CXCl1	_	++	_	+++	_	++
P80162	CXCL6	_	++	_	++	_	++
P42830	CXCL5	_	++	_	++	_	_
P08254	MMP-3/Stromelysin-1	_	+++	_	+++	_	_
P07585	Decorin	++	++++	+++	++++	++	++
P08253	MMP-2	++	++++	+++	+++	++	+++
P09603	CSF-1	_	++	++	++	_	++
P98066	TSG-6	_	++	_	_	_	++
P26022	PTX3/ TSG-14	+++	+++	_	++	+++	++++
P19875	GRO-beta /CXCL2	_	++	_	_	_	_
P05121	PAI-1	+++	+++	++	+++	+++	+++
O00391	Sulfhydryl oxidase 1	(+)	++	++++	+++	_	++
Q15063	Periostin	(+)	_	++	+++	(+)	++
Q9Y240	SCGF	++	_	(+)	++	_	_
Cytoplasm:
O00469	PLOD-2	(+)	++	_	+++	(+)	+++
P05362	ICAM-1 (CD54)	_	_	(+)	+	_	++
P05120	PAI-2	++	+++	_	+++	++	_
Nuclear extract:
Q8WX93	Palladin	++	+++	++	+++	++	+++
P51911	Calponin-1	+++	+++	++	+++	++	+++
Q16666	γ-IFN-inducible protein 16	++	++	++	++	++	+++

The total number of distinct peptides identified per protein when considering all three sub-cellular fractions was used as a semi-quantitative measure for protein abundances in the different fibroblasts, which allowed determining up- or down-regulation of proteins upon inflammatory activation. Most of the regulated proteins were found in the secretomes, listed under “Supernatant”.
AccNr, Swiss-Prot accession number; -, protein was not detected; +, protein was detected with one peptide; ++, protein was detected with 2 to 5 peptides; +++, protein was detected with ≥6 peptides; ++++, protein was detected with ≥15 peptides; (+), protein identification was ambiguous, i.e. protein was not found robustly in all corresponding samples.

ISSN: 1559-0275