Identification of novel protein-coding genes based on peptides mapping to intergenic regions. A) GSSP mapped to intergenic region in Chromosome 1 between CNAG_00389 and CNAG_00390. Gene prediction programs predicted 358 amino acids long proteins from this region, which is homologous to monoubiquitination protein of C. neoformans var. neoformans. B) Three peptides mapped to intergenic region between CNAG_01461 and CNAG_01463. Gene prediction programs predicted protein-coding gene in this region. The predicted protein has homology to retrograde transport, endosome to Golgi related protein, XP_776934.1 (C. neoformans var. neoformans B-3501A), XP_569839.1 (C. neoformans var. neoformans JEC21), XP_003192571.1 (C. gattii WM276). C) The MS/MS spectra of intergenic peptides STPLTVSEDQEDELLSIVPIK and LTFQFITSSEK are shown.