O-GlcNAc profiling: from proteins to proteomes

Table 1 Recognition of antibodies and lectins toward O -GlcNAc (adapted from[29]; Russell Reeves and Natasha E. Zachara, personal communications)

	Reagent name	Antibody isotype	Specificity			Commercial availability	Ref.
	Reagent name	Antibody isotype	O-GlcNAc	β-GlcNAc	GlcNAc*	Commercial availability
Anti-body	CTD110.6	IgM		+		+	[31]
	RL2	IgG	+			+	[32, 33]
	HGAC39	IgG		+			[34]
	HGAC49	IgM		+			[34]
	HGAC85	IgG		+		+	[34]
	9D1.E4(10)	IgG		+		+	[35]
	18B10.C7 (3)	IgG	+			+	[35]
	1 F5.D6(14)	IgG	+			+	[35]
	My95	IgG					[36]
Lectin	WGA/sWGA⁺				+	+	[38]
	GSLII				+	+	[39]

GlcNAc*: all terminal GlcNAc including α/β-GlcNAc.
sWGA⁺: succinated-wheat germ agglutinin. sWGA reduces its affinity for sialic acid and GlcNAc residues. As such, sWGA is typically used for immunoblotting while WGA for purification.

ISSN: 1559-0275