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Table 2 Coefficient of variation (CV) of protein abundance as evaluated using InteQuan and using SISQuan

From: An integrated quantification method to increase the precision, robustness, and resolution of protein measurement in human plasma samples

  Study I Study II Study III Combined HPS dataset
  CV (%) CV (%) CV (%) Generalized CV (%) CV (%) Generalized CV (%)
Protein InteQuan SISQuan InteQuan SISQuan InteQuan SISQuan InteQuan SISQuan InteQuan SISQuan InteQuan SISQuan
KIT 8.8 9.6 4.3 10.5 7.8 35.0 7.7 6.5 10.5 31.7 10.6 12.8
FRIL 16.8 25.2 5.3 11.8 7.3 33.6 7.3 12.0 15.9 25.4 16.7 18.8
COIA1 12.6 17.8 9.0 9.7 10.4 38.5 10.2 14.1 16.1 31.1 16.6 17.5
PRDX1 10.7 15.4 4.9 11.8 9.6 32.2 9.4 14.6 31.9 15.8 31.8 15.5
TENX 11.1 13.6 8.8 12.7 10.7 26.6 10.8 11.1 10.7 32.3 10.6 15.2
ENPL 13.1 18.9 11.2 8.6 11.3 34.9 11.3 13.6 11.7 32.9 11.8 12.6
GRP78 6.8 11.4 19.3 24.2 11.5 33.7 11.2 12.9 13.9 28.5 13.6 18.4
BGH3 5.0 12.3 5.7 9.9 12.4 42.3 12.4 13.8 9.8 33.7 9.6 14.9
ALDOA 6.6 13.6 9.1 17.5 15.1 35.4 14.9 19.9 11.0 35.4 11.2 14.9
GGH 6.9 7.1 9.0 13.7 13.9 38.9 13.7 16.5 11.0 31.7 10.5 16.6
CD14 4.1 8.0 4.6 12.0 4.6 35.3 4.7 6.8 7.9 30.5 7.8 11.9
LG3BP 8.8 13.0 5.9 10.0 5.6 31.1 5.6 7.5 8.6 30.5 8.5 13.9
TSP1 11.6 18.3 12.9 17.0 21.5 45.7 20.9 21.9 20.3 41.1 22.9 25.3
IBP3 5.7 11.6 6.3 13.5 13.5 41.5 14.0 15.5 19.8 26.7 20.5 21.6
TETN 9.9 17.8 9.9 12.4 25.5 52.3 26.9 29.7 33.1 47.6 33.3 37.7
ISLR 10.0 9.1 4.7 10.0 4.2 31.3 4.2 5.0 12.2 33.8 12.1 9.6
Median CV (%) 9.3 13.3 7.6 11.9 11.0 35.1 11.0 13.7 11.9 31.7 11.9 15.3
Median of CV reductiona (%) 4.9   4.8   25.9   2.0   21.0   3.0  
Total proteins with lower CV 15 1 15 1 16 0 15 1 15 1 14 2
P value (paired sign test) 5.2 × 10-4 5.2 × 10-4 3.1 × 10-5 5.2 × 10-4 5.2 × 10-4 4.2 × 10-3
Comments CV of 10 HPS aliquots Median CV of 15 clinical samples Median CV of 6 samples CV of 29 HPS aliquots
  1. aCV reduction was defined as CV using SISQuan minus CV using InteQuan.