Comparison of total protein map and O -GlcNAcylation map of kidney between nondiabetes and diabetes. (A, B) Representative total protein map of 2D PAGE for nondiabetic (A) and diabetic (B) rat kidneys detected by SYPRO-Ruby. (C, D) Typical O-GlcNAcylated protein map of 2D PAGE for nondiabetic (C) and diabetic (D) rat kidneys detected by O-GlcNAc antibody. Spots indicated by arrows represent the identified proteins that had changed in terms of O-GlcNAc level. (E-L) Regions around spots "a," "b," "c," "d," "e," and "f" in the maps "C" and "D" are enlarged to facilitate the identification of each spot, indicated by arrows, in the non-diabetic (E, G, I, K, M, O) and diabetic (F, H, J, L, N, P) kidneys.