Skip to main content

Table 1 Proteins showing an increase in the O-GlcNAcylation level in the diabetic kidney

From: Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4

Spot

Protein

Theoretical mass (kDa)/pI

Score(a)

Peptides

Peptides

matched (a)

Fold changeb)

Sequence

    

searched

 

Wistar(control)

GK

Coverage (%)

a

Actin

42.1/5.29

70

20

8

1

2.15

22

b

α-actinin 4

105.0/5.27

118

40

17

1

2.05

21

c

myosin heavy chain

222.5/5.69

54

18

12

1

2.22

9

d

α-tubulin

50.6/4.95

59

51

9

1

2.75

33

e

ATP-synthase β

56.3/5.19

62

27

9

NDc)

d)

23

f

pyruvate carboxylase

130.4/6.34

73

30

14

1

4.32

16

  1. Spots a-f correspond to those shown in Figure 1.
  2. a) Values of score and peptides matched were determined according to the Mascot search engine on the Swiss-Prot web server (Database: SwissProt 57.6).
  3. b) Fold changes were calculated by using the mean values for the spots of diabetic GK rat kidney relative to those of normal control Wistar rat kidney, which are indicated as "1."
  4. c) ND: not detected.
  5. d) Up-arrow: This O-GlcNAcylated spot appeared only in the diabetic kidney.