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Table 1 Proteins showing an increase in the O-GlcNAcylation level in the diabetic kidney

From: Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4

Spot Protein Theoretical mass (kDa)/pI Score(a) Peptides Peptides matched (a) Fold changeb) Sequence
     searched   Wistar(control) GK Coverage (%)
a Actin 42.1/5.29 70 20 8 1 2.15 22
b α-actinin 4 105.0/5.27 118 40 17 1 2.05 21
c myosin heavy chain 222.5/5.69 54 18 12 1 2.22 9
d α-tubulin 50.6/4.95 59 51 9 1 2.75 33
e ATP-synthase β 56.3/5.19 62 27 9 NDc) d) 23
f pyruvate carboxylase 130.4/6.34 73 30 14 1 4.32 16
  1. Spots a-f correspond to those shown in Figure 1.
  2. a) Values of score and peptides matched were determined according to the Mascot search engine on the Swiss-Prot web server (Database: SwissProt 57.6).
  3. b) Fold changes were calculated by using the mean values for the spots of diabetic GK rat kidney relative to those of normal control Wistar rat kidney, which are indicated as "1."
  4. c) ND: not detected.
  5. d) Up-arrow: This O-GlcNAcylated spot appeared only in the diabetic kidney.