Desmin immunofluorescence staining. Representative examples of desmin staining with Alexa 488 (green) conjugated secondary antibody, and DAPI nuclei stain (blue) performed on colon tissues. A, tumor section showing stromal desmin staining. B, enlargement of white boxed area in A showing desmin positive cells surrounding a crypt. C, from the matched normal tissue section showing desmin-positive muscularis mucosa across the top of the section. Note the much reduced desmin expression in the normal epithelial tissue. Scale bar = 100 μm.