Desmin and von Willebrand factor (VWF) immunostaining. Dual immunostaining performed on stage III tumor tissue. Desmin was visualised with an Alexa 488 (green) conjugated secondary and VWF with an Alexa 568 (red) conjugated secondary, counterstained with DAPI (blue), scale bar = 100 μm. Co-localisation of the proteins appears yellow. Red arrows indicate cells positive for VWF, white arrows cells positive for desmin and yellow arrows co-localisation for desmin and VWF. The tumor section shows a blood vessel outlined by cells showing co-localisation of desmin (pericytes) and VWF (endothelial cells) in juxtaposition.