Immunohistochemical labeling of AMPH protein overexpressed in TBM. Normal controls (A-C): Whole mount preparation of cerebral cortex (A) shows labeling of cortical ribbon in a synaptic pattern (B). Higher magnification highlights scattered strong labeling of pyramidal neurons (arrow, C) with synaptic pattern of neuropil labeling. TBM (D-F): Strong labeling of the cortical ribbon seen in contrast to controls (D) with intense labeling of neuropil in grey matter (E) and pyramidal neurons in the cortex (F). [A, D: whole mounts x10, B, E: Obj x5, C: Obj x40, F: Obj x 20] [g – grey matter, w – white matter].