Figure 3

Hierarchy of protein correlations across sample set. This depicts the unsupervised hierarchical clustering of proteins identified in the CSF proteome based on protein abundance Pearson's cross correlation coefficient. Protein abundance patterns are either positively correlated (red) or anti-correlated (green). Pathway analysis of clusters revealed that cluster A was composed of proteins that were enriched with respect to functions of wound healing, adaptive immunity, and acute phase signaling. Cluster A could be further separated into two regions depicted as clusters 1 and 2 with strong positive correlation with annotated functional enrichment of wound healing and immune response respectively. Cluster C contains two regions of strong internal correlation with annotated functional enrichment of alternate complement cascade and cell adhesion. Interestingly the proteins in cluster A were largely anti-correlated with proteins in cluster C, and had functional attributes referable to complement and acute phase response as well as cell movement.