Figure 4

Cross correlation analysis of proteins quantified in the CSF proteome and orthogonal markers of CNS immune activation (neopterin), neural injury (NFL) and infection (HIV RNA). A-C plot the distributions of cross correlation coefficients or proteins with these three CSF biomarkers while D-F plot the proteins with significant correlations (R ≤ -0.3 or ≥ 0.3 and p ≤ 0.05) with the respective CSF biomarkers plotted against their respective protein fold changes determined by MS as measured over the course of ART. The heat map (G) that includes the cross correlations of proteins correlating with three orthogonal analytical measurements of disease progression shows that distinctly different correlation patterns result from hierarchical clustering of all protein cross correlation values. The Venn diagram (H) of proteins that show significant correlations (R ≥ 0.3 or ≤ -0.3, and p ≤ 0.05) with neopterin (red, n = 99), NFL (blue, n = 66) and CSF HIV RNA (green, n = 9) shows the degree of overlap of correlating proteins.