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Figure 1 | Clinical Proteomics

Figure 1

From: Selected Reaction Monitoring (SRM) Analysis of Epidermal Growth Factor Receptor (EGFR) in Formalin Fixed Tumor Tissue

Figure 1

Quantitative analysis of the epidermal growth factor receptor (EGFR) protein by enzyme-linked immunosorbent assay (ELISA) sandwich immunoassay in cultured cell lines as a basis for comparison with the mass spectrometry-based Selected Reaction Monitoring (SRM) analysis. (A) Serial dilutions of a recombinant EGFR standard analyzed in triplicate by ELISA to establish a quantitative curve for comparison with EGFR levels from cell lines. (B) Plot of these data on a linear regression curve (R2 = 0.9869) (C) ELISA immunoanalysis of EGFR from protein lysates from non-fixed cells derived from 4 cell lines. (D) Plot of these data on a bar graph demonstrating a quantitative range of EGFR protein in the cell lines. CV = coefficient of variance.

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Clinical Proteomics

ISSN: 1559-0275