Figure 2

Analysis of EGFR using mass spectrometry-based SRM (EGFR-SRM) of Liquid Tissue lysates from the formalin fixed paraffin embedded (FFPE) culture cells compared with ELISA analysis for EGFR from matching non-fixed cells. (A) Standard SRM curve generated using unlabeled and isotopically labeled synthetic peptide that is used to define the limit of detection (LOD, 31 amol), define the limit of quantitation (LOQ, 62amol), and determine the linearity of the assay (R2 = 0.9994). (B) Triplicate EGFR-SRM analysis of Liquid Tissue lysates from formalin fixed cells. Quantitative data is reflected in amol/μg total protein. (C) Bar graph analysis of EGFR-SRM data. (D) Linear regression curve demonstrating correlation (R2 = 0.9991) between quantitative EGFR as determined by ELISA (EGFR-ELISA) analysis of non-fixed cells and quantitative EGFR-SRM analysis of the matching formalin fixed cells. SD = standard deviation.