Figure 7

Differentially detected host and pathogen proteins of B. mallei infection. (A) Heatmap of IgG interactions from Chlorocebus aethiops sera (individuals in columns) with microarrayed B. mallei proteins (in rows). (B) Antibody responses (±SD) to GroEL in comparison to controls were significantly elevated (p ≤ 0.05) in serum collected after infection with B. mallei (middle panel) from four Chlorocebus aethiops monkeys that succumbed to infection, on days 5 (ID 1), 11 (ID 2), 13 (ID 3), 14 (ID 4); and in serum collected on 28 from one monkey that recovered from infection (ID 5). There were no measurable antibody responses from any of the monkeys to the control proteins y1030 and y1025 from the closely related bacterium Yersinia pestis (lower panel). (C) Venn diagram representing the number of qualitative host protein changes observed in the lung (35), the skin (125), and overlapping (15). The proteins overlapping in the Venn diagram are presented in Table 1.