This shows the change in immunohistochemistry intensity between primary tumours and lymph node metastasis. Wilcoxon signed-rank test for paired samples were performed to assess statistical significance. Red line represents the mean. a) The expression of ATPase inhibitory factor 1 significantly decrease (p = 2.8e−09). b) The expression of tubulin β-chain significantly decreases (p = 3.5e−05).