Figure 6

Differentially synthesized proteins. A) Proteins with different abundance levels between the two groups, as determined by label-free quantification after LC-MS. Quantitative values shown correspond to average values between the total samples in which a given protein was identified (ACAN: OA = 9, control = 6; COL2A1: OA = 5, control = 5; TG2: OA = 10, control = 6; CTSB: OA = 10, control = 6; CTNNB1: OA = 9, control = 0; HSD17B12: OA = 9, control = 0; PLA2G2A: OA = 5, control = 5; APCS: OA = 4, control = 5; PRDX2: OA = 10, control = 6; GSTP1: OA = 10, control = 6; PLS3: OA = 10, control = 5; SERPINH1/HSP47: OA = 9, control 6; MVP: OA = 10, control = 6; MYOF: OA = 10, control = 6). Error-bars correspond to ±1 s.e. B) Western blot analysis for the validation of label-free quantication method, using antibodies against GSTP1 and PLS3 in four different OA and control samples. Values are normalized to β-actin. Error-bars correspond to ±1 s.e. C) Quantitation (arbitrary units) of GSTP1 per sample. D) Quantitation (arbitrary units) of PLS3 per sample E) Quantitation (arbitrary units) of β-actin per sample.