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Fig. 1 | Clinical Proteomics

Fig. 1

From: Syringe-push membrane absorption as a simple rapid method of urine preparation for clinical proteomics

Fig. 1

Silver Blue G-250 staining [12] showed deep blue staining of absorbed proteins on nitrocellulose and PVDF, but not Whatman no.1 membranes (a). Absorbed proteins were eluted by 2-D lysis buffer and measured by the Bradford protein assay for recovery yield and inter-CV (b). Eluted proteins were subsequently analyzed by 2-DE (50 μg/gel) with silver blue G-250 staining (c). All experiments were performed in triplicate

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