Fig. 2

Strategy for selection of candidates for Western blot analysis. Potential candidates were initially selected from the set of 320 proteins identified by MS of radical prostatectomy sections by comparison with proteins annotated for PCa in the Human Protein Atlas (HPA, http://www.proteinatlas.org) and then further selected by literature searches for known associations with PCa (Additional file 6). A refined set of four candidates was examined by Western blotting of archival FFPE specimens for prediction of risk of metastatic disease