Fig. 1
Selection of lectins for capturing human salivary N-glycosites. a The electrophoretic banding patterns of submandibular/sublingual (SMSL) and parotid (PS) salivary proteins and glycoproteins as visualized by Coomassie blue or Alcian blue silver staining, respectively. Replicate blots were screened against a panel of 16 lectins (Additional file 1: Figure S1), 4 of which are shown in this figure (b, c). The patterns of reactive bands for three lectins, which in the aggregate, reacted with salivary components over a broad molecular weight range (AAL, JAC and WGA) are shown. LEA was selected due to its reactivity with extended lactosamine units, potential sites of terminal saccharide modifications related to blood group status