Fig. 6

Lectin capture efficiency, in terms of number of N-glycosites identified and relative abundance. Results shown are of analysis of a single secretor and a single nonsecretor. a (upper) Overall, AAL enrichment tended to yield the greatest number of N-glycosites. a (middle) With regard to N-glycosites that were common among donors, lectin performance did not depend on secretor status (a, lower) whereas more N-glycosites tended to be captured by AAL from the nonsecretor sample. b (upper) Overall, AAL demonstrated the greatest capture efficiency, enriching more N-glycosites from the parotid saliva sample of the nonsecretor (starred). b (middle) With regard to N-glycosites that were common among donors, the low level of jacalin capture of parotid sites from the secretor sample was evident (starred). b (lower) With regard to donor-unique N-glycosites, AAL capture from parotid saliva of the nonsecretor was once again most productive in terms of a number of identified N-glycosites (starred). c (upper) As to relative abundances in terms of spectral counts and overall performance, WGA and AAL capture tended to have the highest efficiently. c (middle) In terms of donor-common species, the highest number of N-glycosites tended to be found in the AAL bound fraction of the secretor parotid saliva sample. c (lower) In terms of donor-unique sites, WGA captured the highest numbers from the nonsecretor parotid saliva sample (starred)