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Fig. 2 | Clinical Proteomics

Fig. 2

From: Phosphotyrosine profiling of curcumin-induced signaling

Fig. 2

Workflow employed to identify changes in the phosphorylation status in response to curcumin. CAL 27 cells were cultured in “light” or “heavy” SILAC medium. The cells grown in ‘light’ medium were treated with curcumin for 4 h and the cells grown in ‘’heavy medium’’ were treated with vehicle (DMSO). The samples were subjected to trypsin digestion and enriched for phosphopeptides using anti-phosphotyrosine antibodies for enrichment of tyrosine-phosphorylated peptides. The enriched phosphopeptides were analyzed by LC-MS/MS

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