Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation

Rawat, Preeti; Bathla, Shveta; Baithalu, Rubina; Yadav, Munna Lal; Kumar, Sudarshan; Ali, Syed Azamal; Tiwari, Anurag; Lotfan, Masoud; Naru, Jasmine; Jena, Manoj; Behere, Pradip; Balhara, Ashok K.; Vashisth, Rajesh; Singh, Inderjeet; Dang, Ajay; Kaushik, Jai K.; Mohanty, Tushar K.; Mohanty, Ashok K.

doi:10.1186/s12014-016-9116-y

Clinical Proteomics

Table 4 List of down regulated proteins

From: Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation

S. no.	Protein ID	Protein name	Peptides	Sequence coverage (%)	Mol. weight (kDa)	Fold change
S. no.	Protein ID	Protein name	Peptides	Sequence coverage (%)	Mol. weight (kDa)	16 vs 0 days	22 vs 0 days	35 vs 0 days
1	gi\|594080166	Antithrombin-III	3	1.9	52.4	0.3	0.4	0.5
2	gi\|741962906	Sex hormone-binding globulin isoform X2	11	44.8	37.3	0.2	0.6	0.5
3	gi\|358420932	Serpin B4-like	8	21.7	44.3	0.1	0.0	0.0
4	gi\|57619329	Actin, cytoplasmic 1	9	33.9	41.7	0.1	0.4	0.2
5	gi\|803211084	Nuclear transport factor 2	3	45.7	14.4	0.07	0.07	0.7
6	gi\|371491843	Peptidoglycal recognition protein 1	6	40	21	0.03	0.0	0.0
7	gi\|239919016	Immunoglobulin G3 heavy chain constant region, partial	2	7.3	25	0.34	0.1	4.3
8	gi\|109030	Ig lambda chain C region - (fragment)	2	18.1	11.311	0.03695	0.29332	0.04130
9	gi\|927774498	A Chain A, Bovine Allergen Bos D 2 In The Monoclinic Space Group C2	7	44.2	17.8	0.5	0.09	0.0
10	gi\|194206368	OTU domain-containing protein 7A	2	1	79.606	0.56012	0.63301	0.60267
11	gi\|664707994	EGF-containing fibulin-like extracellular matrix protein 1 isoform X1	11	30.1	54.8	0.2	0.6	0.4
12	gi\|803030626	Glutaminyl-peptide cyclotransferase isoform X1	8	37.7	41	0.3	0.5	0.5
13	gi\|594074072	Ligand isoform X3	2	3.6	0.4	0.6	0.4	0.4
14	gi\|803074446	Myeloblastin	2	4.5	24.258	0.10620	0.30618	0.18733
15	gi\|803156785	Signal peptide, CUB and EGF-like domain-containing protein 2 isoform X4	2	2	84.1	0.6	0.5	0.2
16	gi\|741886482	Neutrophil gelatinase-associated lipocalin	7	42.7	22.8	0.1	0.01	0.02
17	gi\|803333753	Annexin A1	12	39.9	38.8	0.2	0.3	0.2
18	gi\|803284484	Transmembrane glycoprotein NMB	4	6.5	61.6	0.1	0.6	0.5
19	gi\|594070987	Pantetheinase isoform X6	5	17	49.9	0.08	0.1	0.3
20	gi\|803234439	Alpha/beta hydrolase domain-containing protein 14B	3	17.1	22.4	0.09	0.5	0.3
21	gi\|803299355	Cadherin-19 isoform X1	2	2.6	86.8	0.5	0.6	0.3
22	gi\|440901131	Protein AMBP, partial	6	13.5	37.2	0.4	0.5	0.5
23	gi\|803219892	Endothelial protein C receptor isoform X4	5	28.6	24.7	0.3	0.6	0.5
24	gi\|594051858	Cartilage intermediate layer protein 1 isoform X8	6	6.2	123.9	0.0	0.3	0.1
25	gi\|594114749	Kallikrein-1	5	42.9	28.7	0.1	0.3	0.4
26	gi\|440904881	Dermatopontin	8	51.3	23.7	0.1	0.4	0.3
27	gi\|440905390	Haptoglobin, partial	6	18.5	44.7	0.02	0.05	0.02
28	gi\|440909177	Cubilin, partial	12	4.2	396.7	0.01	0.1	0.01
29	gi\|594044657	Resistin	2	12.8	11.4	0.1	0.09	0.2
30	gi\|440913634	Leukocyte elastase inhibitor	4	11.6	45.1	0.05	0.08	0.03
31	gi\|594086536	Collagen alpha-3(VI) chain isoform X4	6	2.8	325.1	0.1	0.4	0.5
32	gi\|741942660	EGF-containing fibulin-like extracellular matrix protein 1 isoform X1	14	35.9	54.8	0.2	0.4	0.5
33	gi\|528978722	Factor XIIa inhibitor isoform X1	7	20.5	51.7	0.02	0.3	0.2
34	gi\|529002050	Lactotransferrin isoform X1	25	37.9	78.0	0.1	0.4	0.05
35	gi\|664770863	Dipeptidyl peptidase 2	2	4.9	54.5	0.2	0.6	0.2
36	Q3SZV7	Q3SZV7 Similar to hemopexin	4	14.8	52.2	0.06	0.4	0.1
37	gi\|594103691	Allergen Bos d 2-like	2	11.1	18.9	0.1	0.1	0.1
38	gi\|741973323	Serpin B3-like isoform X2	5	11.5	48.1	0.08	0.1	0.2
39	gi\|440895590	Growth arrest-specific protein 7, partial	2	0	35.4	0.03	0.15	0.1
40	gi\|545223253	Teneurin-4	2	0	277.6	0.2	0.3	0.3

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