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Table 2 Recovery and coefficients of variation (CVs) of 3 pmol of BSA protein and its proteotypic peptides in technical triplicates of two urine samples, utilizing four methods as described in the text

From: Quantification of angiotensin II-regulated proteins in urine of patients with polycystic and other chronic kidney diseases by selected reaction monitoring

Protein/peptides Mean recovery—urine 1 (pmol)a CV (%) Mean recovery—urine 2 (pmol)a CV (%)
Method 1Amicon/LysC-Tryp     
BSA     
 LVNELTEFAK 6.137 ± 0.124 2.0 5.011 ± 0.08 1.7
 HLVDEPQNLIK 6.242 ± 0.058 0.9 4.852 ± 0.059 1.2
 LGEYGFQNALIVR 3.046 ± 0.056 1.8 2.770 ± 0.030 1.1
Method 2Amicon/Tryp     
BSA     
 LVNELTEFAK 2.061 ± 0.017 0.8 1.36 ± 0.064 4.7
 HLVDEPQNLIK 2.317 ± 0.071 3.1 1.52 ± 0.037 2.4
 LGEYGFQNALIVR 1.308 ± 0.011 0.8 1.044 ± 0.012 1.1
Method 3Acetonitrile/LysC-Tryp     
BSA     
 LVNELTEFAK 4.400 ± 0.029 0.7 3.980 ± 0.213 5.3
 HLVDEPQNLIK 4.568 ± 0.119 2.2 3.984 ± 0.058 1.5
 LGEYGFQNALIVR 1.768 ± 0.026 1.5 2.124 ± 0.013 0.6
Method 4—Acetonitrile/Tryp     
BSA     
 LVNELTEFAK 2.00 ± 0.005 0.2 1.950 ± 0.010 0.5
 HLVDEPQNLIK 3.46 ± 0.098 2.9 2.280 ± 0.130 5.8
 LGEYGFQNALIVR 0.592 ± 0.005 0.9 1.535 ± 0.011 0.7
  1. aMean ± SD