Quantification of angiotensin II-regulated proteins in urine of patients with polycystic and other chronic kidney diseases by selected reaction monitoring

Table 2 Recovery and coefficients of variation (CVs) of 3 pmol of BSA protein and its proteotypic peptides in technical triplicates of two urine samples, utilizing four methods as described in the text

Protein/peptides	Mean recovery—urine 1 (pmol)^a	CV (%)	Mean recovery—urine 2 (pmol)^a	CV (%)
Method 1—Amicon/LysC-Tryp
BSA
LVNELTEFAK	6.137 ± 0.124	2.0	5.011 ± 0.08	1.7
HLVDEPQNLIK	6.242 ± 0.058	0.9	4.852 ± 0.059	1.2
LGEYGFQNALIVR	3.046 ± 0.056	1.8	2.770 ± 0.030	1.1
Method 2—Amicon/Tryp
BSA
LVNELTEFAK	2.061 ± 0.017	0.8	1.36 ± 0.064	4.7
HLVDEPQNLIK	2.317 ± 0.071	3.1	1.52 ± 0.037	2.4
LGEYGFQNALIVR	1.308 ± 0.011	0.8	1.044 ± 0.012	1.1
Method 3—Acetonitrile/LysC-Tryp
BSA
LVNELTEFAK	4.400 ± 0.029	0.7	3.980 ± 0.213	5.3
HLVDEPQNLIK	4.568 ± 0.119	2.2	3.984 ± 0.058	1.5
LGEYGFQNALIVR	1.768 ± 0.026	1.5	2.124 ± 0.013	0.6
Method 4—Acetonitrile/Tryp
BSA
LVNELTEFAK	2.00 ± 0.005	0.2	1.950 ± 0.010	0.5
HLVDEPQNLIK	3.46 ± 0.098	2.9	2.280 ± 0.130	5.8
LGEYGFQNALIVR	0.592 ± 0.005	0.9	1.535 ± 0.011	0.7

ISSN: 1559-0275