Table 3 Deamidated peptides monitored and their contribution to the overall peptide areas and ratios
Protein/peptide | L/H ratio formula (non-deamidated formula on top, total on bottom) | Heavy peptide deamidation  % (mean ± SD) | Light peptide deamidation  % (mean ± SD) | Mean peptide  % Conc. ∆ due to deamidation |
|---|---|---|---|---|
TSP1/GGVNDNFQGVLQNVR | \(\frac{808.9}{813.9}\) | 33 ± 1.2 | 39 ± 6.5 | 6.5 |
\(\frac{808.9 + 809.4 + 809.4 + 809.4}{813.9 + 814.4 + 814.4 + 814.4}\) | ||||
BST1/GFFADYEIPNLQK | \(\frac{771.3}{775.3}\) | 35 ± 0.8 | 44 ± 3.8 | 12 |
\(\frac{771.3 + 771.8 + 771.8 + 772.3}{775.3 + 775.8 + 775.8 + 776.3}\) | ||||
TSP1/TIVTTLQDSIR | \(\frac{623.8}{628.8}\) | 28 ± 0.3 | 33 ± 3.7 | 7 |
\(\frac{623.8 + 624.3}{628.8 + 629.3}\) | ||||
RHOB/IQAYDYLECSAK | \(\frac{730.8}{734.8}\) | 20 ± 0.9 | 15 ± 14.8 | 12 |
\(\frac{730.8 + 731.3}{734.8 + 735.3}\) |