Skip to main content

Advertisement

Table 3 Deamidated peptides monitored and their contribution to the overall peptide areas and ratios

From: Quantification of angiotensin II-regulated proteins in urine of patients with polycystic and other chronic kidney diseases by selected reaction monitoring

Protein/peptide L/H ratio formula (non-deamidated formula on top, total on bottom) Heavy peptide deamidation  % (mean ± SD) Light peptide deamidation  % (mean ± SD) Mean peptide  % Conc. ∆ due to deamidation
TSP1/GGVNDNFQGVLQNVR \(\frac{808.9}{813.9}\) 33 ± 1.2 39 ± 6.5 6.5
\(\frac{808.9 + 809.4 + 809.4 + 809.4}{813.9 + 814.4 + 814.4 + 814.4}\)
BST1/GFFADYEIPNLQK \(\frac{771.3}{775.3}\) 35 ± 0.8 44 ± 3.8 12
\(\frac{771.3 + 771.8 + 771.8 + 772.3}{775.3 + 775.8 + 775.8 + 776.3}\)
TSP1/TIVTTLQDSIR \(\frac{623.8}{628.8}\) 28 ± 0.3 33 ± 3.7 7
\(\frac{623.8 + 624.3}{628.8 + 629.3}\)
RHOB/IQAYDYLECSAK \(\frac{730.8}{734.8}\) 20 ± 0.9 15 ± 14.8 12
\(\frac{730.8 + 731.3}{734.8 + 735.3}\)
  1. Mean % of heavy peptide deamidations, % of light peptide deamidations and % change in concentration (due to the modified forms) for each peptide, and across all urine samples is shown