Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations

Table 1 Masses of analyte and AQUA standard peptides used for quantitation of GP₁ in eVLP

Set	Sequence	Analyte		AQUA standard
Set	Sequence	2(+) m/z	3(+) m/z	2(+) m/z	3(+) m/z
1	SEELSFTAVSNR	670.3281	447.2211	675.3322	450.5572
1	IRSEELSFTAVSNR	804.9206	536.9495	809.9248	540.2856
2	SVGLNLEGNGVATDVPSATK	964.9998	643.669	969.0069	646.3404
2	SVGLNLEGNGVATDVPSATKR	1043.0498	695.7027	1048.0549	699.0388
2	SVGLNLEGNGVATDVPSATK-deam	965.4918	643.997	N/A	N/A
2	SVGLNLEGNGVATDVPSATKR-deam	1043.5424	696.0307	N/A	N/A

ISSN: 1559-0275