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Table 2 HR/AM-MS method validation using purified recombinant GP1 standard

From: Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations

Trial

Dilution

Peptide

On-column

Total In sample (µg)

Ave. (µg)

ΔS (%)

Accuracy (%)

Precision (% CV)

fmoles

ng

1

80

Set 1

347.7

17.7

1.42

1.45

4.60

80.5

3.2

Set 2

363.7

18.5

1.43

2

80

Set 1

363.3

18.5

1.48

1.52

5.50

84.5

Set 2

383.4

19.5

1.56