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Table 2 HR/AM-MS method validation using purified recombinant GP1 standard

From: Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations

Trial Dilution Peptide On-column Total In sample (µg) Ave. (µg) ΔS (%) Accuracy (%) Precision (% CV)
fmoles ng
1 80 Set 1 347.7 17.7 1.42 1.45 4.60 80.5 3.2
Set 2 363.7 18.5 1.43
2 80 Set 1 363.3 18.5 1.48 1.52 5.50 84.5
Set 2 383.4 19.5 1.56
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Clinical Proteomics

ISSN: 1559-0275