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Table 3 GP1 quantitation in three replicates of a single eVLP lot

From: Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations

Sample

Digest (µg)

fmoles

Aliquot (µg)

Concentration (mg/mL)

Pct. total protein

∆S (%)

CV%

Pep Set 1

Pep Set 2

Lot A (1)

10

199.1

231.1

1.22

0.57

12.2

16.1

7.60 %

Lot A (2)

10

172.3

207.1

1.05

0.49

10.5

20.2

Lot A (3)

10

183.8

212.2

1.12

0.53

11.2

15.5

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Clinical Proteomics

ISSN: 1559-0275