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Table 4 LC-HRMS results of GP1 quantitation in 5 lots of eVLP and comparison with quantitative western blot values

From: Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations

Lot Concentration (mg/mL) MS (CV%) Average ∆S (%) GP1 %TP
Protein rGP WB MS
A 4.7 1.0 0.55 6.0 % 15.8 11.7
B 3.3 1.14 0.33 9.9 % 7.35 8.6
C 4.8 1.4 0.29 11.6 % 12.7 6.1
D 4.9 0.7 0.15 7.2 % 24.1 3.0
E 7.2 1.1 0.09 5.2 % 25.5 1.2