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Table 4 LC-HRMS results of GP1 quantitation in 5 lots of eVLP and comparison with quantitative western blot values

From: Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations

Lot

Concentration (mg/mL)

MS (CV%)

Average ∆S (%)

GP1 %TP

Protein

rGP WB

MS

A

4.7

1.0

0.55

6.0 %

15.8

11.7

B

3.3

1.14

0.33

9.9 %

7.35

8.6

C

4.8

1.4

0.29

11.6 %

12.7

6.1

D

4.9

0.7

0.15

7.2 %

24.1

3.0

E

7.2

1.1

0.09

5.2 %

25.5

1.2

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Clinical Proteomics

ISSN: 1559-0275