| db/db mouse model | STZ mouse model | |||||
---|---|---|---|---|---|---|---|
Control db/+ (n = 5) | db/db (n = 5) | Insulin db/db (n = 5) | P valueA | Control (STZ) (n = 6) | STZ (n = 6) | P valueB | |
 | 10 weeks old, baseline (insulin dosing start) | 10 weeks old, baseline, 2 weeks after STZ | |||||
BG (mM) | 8.85 ± 0.48 | 19.27 ± 2.29 | 24.34 ± 1.28 | <0.002§ <0.001$ | 6.50 ± 0.27 | 22.1 ± 1.60 | <0.001 |
HbA1c (%) | 4.06 ± 0.08 | 6.44 ± 0.48 | 7.88 ± 0.44 | <0.003§ <0.001$ <0.05€ | – | – | NA |
AER (μg/24 h) | 42.5 ± 9.8, n = 4 | 420.8 ± 111.9 | 529.3 ± 115 | <0.017§ <0.06$ | 40.8 ± 16.2, n = 5 | 408 ± 114 | <0.033 |
BW (g) | 27.1 ± 0.9 | 46.66 ± 0.5 | 47.92 ± 0.2 | <0.001§,$ | 25.2 ± 1.1 | 23.6 ± 0.4 | ns |
 | 16 weeks old, 6 weeks after insulin dosing start | 14 weeks old, 6 weeks after STZ | |||||
BG (mM) | 8.4 ± 0.33 | 22.88 ± 1.86 | 16.14 ± 1.61 | <0.001§ <0.007$ <0.017€ | 5.63 ± 0.25 | 30.9 ± 3.19 | <0.001 |
HbA1c (%) | 4.16 ± 0.10 | 7.94 ± 0.62 | 7.32 ± 0.23 | <0.001§,$ | 3.62 ± 0.031 | 6.95 ± 0.1 | <0.001 |
AER (μg/24 h) | 59.4 ± 13.3, n = 4 | 855.3 ± 272 | 488.6 ± 61.2 | <0.024§ | 55.6 ± 5.39, n = 5 | 424 ± 71 | <0.012 |
BW (g) | 31 ± 0.8 | 54 ± 3.2 | 57.2 ± 1.1 | <0.001§,$ | 28.1 ± 1.0 | 24.8 ± 0.5 | <0.018 |
 | 22 weeks old, 12 weeks after insulin dosing start | 18 weeks old, 10 weeks after STZ | |||||
BG (mM) | 8.91 ± 0.28 | 23.12 ± 2.54 | 21.23 ± 1.49 | <0.001§,$ | 8.57 ± 1.21 | 33.4 ± 1.45 | <0.001 |
HbA1c (%) | 4.3 ± 0.07 | 8.52 ± 0.70 | 6.78 ± 0.23 | <0.001§ <0.005$ <0.035€ | 3.75 ± 0.06 | 7.60 ± 0.13 | <0.001 |
AER (μg/24 h) | 112.2 ± 54.5, n = 4 | 930.2 ± 184.8 | 795.1 ± 183.8 | <0.015§ <0.039$ | 55.7 ± 12.0, n = 5 | 578 ± 117 | <0.004 |
BW (g) | 32.7 ± 0.7 | 53.7 ± 3.8 | 62.3 ± 0.9 | <0.001§,$ <0.05€ | 30 ± 0.5 | 25.3 ± 1.2 | <0.007 |