N-glycosylation proteome enrichment analysis in kidney reveals differences between diabetic mouse models

Table 2 Selected proteins identified in both mouse models with opposite or similar protein regulation

Uniprot ID	Description	Short name	STZ versus NoSTZ		db/db versus db/+ db/db insulin versus dbdb^B		Kidney Compartment^C
Uniprot ID	Description	Short name	Protein abuncance level	P value	Protein abundance level	P value	Kidney Compartment^C
P09055	Integrin beta-1	ITGB1	Up	<0.012	Down	<0.002	G = H, T = L
Q8C3K6	Sodium/glucose cotransporter 1	SGLT1	Down	<0.018	Up	<0.008	T = M
O54990^A	Prominin-1	PROM1	Down	<0.049	Up	<0.001	T = H
Q3TQ02	Alkaline phosphatase, unreviewed	ALPL	Down	<0.037	Up	<0.001	T = M
Q3TA96	Lysosomal membrane glycoprotein 1, unreviewed	LAMP1	Up	<0.011	Up	<0.018	G = H, T = H
Q07797	Galectin-3-binding protein	GAL-3BP	Up	<0.001	Up	<0.001	G = L, T = L
Q62470-2	Integrin alpha-3, isoform 2	ITGA3	Up	<0.03	Up	<0.001	G = M, T = H
Q8QZW3	Family with sequence similarity 151, member A	F151A	Down	<0.001	Down Up^B	<0.011 <0.01^B	T = H
A0PJK7	Cadherin-related family member 5, unreviewed	CDHR5	Down	<0.016	Down Tr up^B	<0.006 <0.099^B	T = H
Q61847-2	Meprin A subunit beta, isoform 2	MEP1B	Down	<0.008	Down	<0.002	ND, RNA ND
Q6IR20	Solute carrier organic anion transporter family, member 1a6, unreviewed	SLC1A6	Down	<0.030	Down Up^B	<0.001 <0.032^B	G = L, T = L

11 of the 27 VIP proteins had significantly different abundances in both the STZ and the db/db mouse models. Uniprot accession number, name of the protein and a short name is shown for all included proteins together with the direction of change in protein abundance level in the diabetic mice compared to the healthy mice within each mouse model and the P value. ^AIn PROM1, there was significant difference in intragroup variance. Two-tailed Student’s t test for equal variance was used for P value calculations except for the cases where insulin had an effect on the protein level, indicated by^B. One-way ANOVA with Tukey post hoc test for correction of multiple comparisons was used for db/db insulin and db/db vehicle comparisons. P < 0.05 was considered significant. ^CKidney compartments were obtained from The Human Protein Atlas [28] using antibody staining where G glomerulus, T tubulus, ND no detected protein, L low, M medium, H high protein expression, RNR means that RNR was detected in the indicated compartment but no protein expression has been confirmed. Tr trend, with P < 0.1, > 0.05

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