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Table 4 Insulin effect on protein abundance in the db/db mouse model

From: N-glycosylation proteome enrichment analysis in kidney reveals differences between diabetic mouse models

Uniprot ID

Description

Short name

db/db versus db/+

db/db insulin versus db/db

Kidney compartmentB

Protein abundance level

P value

Protein abundance level

P value

Away from healthy control

P19137/F8VQ40A

Laminin subunit alpha 1

LAMA1

Down

<0.039

Down

<0.006

G = M, T = H

G5E8Q8

Adhesion G protein-coupled receptor 116

GPR116

Down

<0.019

Down

<0.027

ND, RNA T = H, G = L

E9Q3Q6

CD166 antigen, activated leukocyte cell adhesion molecule, unreviewed

ALCAM

Up

<0.018

Up

<0.033

T

Insulin increases protein abundance towards healthy control

Q3TDY6

Tripeptidyl peptidase I, PUP

TPP1

Down

<0.005

Up

<0.005

G = L, T = H

A2ARV4

Low-density lipoprotein receptor-related protein 2

LRP2

Down

<0.001

Up

<0.023

T

Q8VBZ3

Cleft lip and palate associated transmembrane protein 1

CLPT1

Down

<0.001

Up

<0.01

ND, RNA T = H, G = L

Insulin reduces protein abundance towards healthy control

Q9JKR6

Hypoxia up-regulated protein 1

HYOU1

Up

<0.081

Down

<0.029

ND, RNA T

Q1MXF9

Sodium channel beta4 subunit, unreviewed

SCN4B

Up

<0.001

Down

<0.001

G = L, T = M

Q3U4F3

Acid phosphatase 2, lysosomal, PUP

ACP2

Up

<0.019

Down

<0.004

ND, RNA T

A2AKI5

Integrin alpha-V light chain, unreviewed

ITGAV

Up

<0.001

Down

<0.016

G = H, T = M

Q3UP74

Alanyl (membrane) aminopeptidase, PUP

ANPEP

Up

<0.001

Down

<0.047

T

Q9JHJ8

Inducible T-cell co-stimulator ligand

ICOSL

Up

<0.001

Down

<0.007

G = L, T = M

  1. In addition to the effect insulin had on some of the 27 VIP proteins, the abundances of 12 of the 153 proteins identified in both mouse models were affected by insulin. The protein abundance level shown as up or down and the P values are valid for the first group compared to the second group (db/db compared to the db/+ and db/db insulin compared to db/db respectively). Protein data is reported as in Table 2, but the STZ mouse data is not shown, as there were no significant differences except for in LAMA1. ALAMA1 was identified in the STZ mouse model solely with Uniprot accession P19137 and in the db/db mouse model solely with Uniprot accession F8VQ40 (unreviewed). The sequences share 99.7 % identity and differ in length by 1 amino acid (aa) (3084 and 3083 aa respectively). The abundances of P19137 and F8VQ40 were assumed comparable. BThe kidney compartment reported in the last column corresponds to the findings reported in the Human Protein Atlas [28]. One-way ANOVA with Tukey post hoc test for multiple comparison correction was used for P value calculations, P < 0.05 was considered significant. PUP putative uncharacterized protein