Fig. 1
From: Plasma degradome affected by variable storage of human blood
Experimental workflow. Four EDTA blood tubes were collected from five healthy volunteers (n = 5) and remained at ambient temperature for T = 30 min, 8, 24 or 48 h before centrifugation, processing and analysis by liquid chromatography tandem mass spectrometry (LC–MS/MS). Comparison of plasma proteomic signatures of individual samples by LC–MS/MS of tryptic (reflecting proteins) and naturally occurred peptides (peptidome) in blood performed at the indicated time points. Profiling of protein degradation (degradome) in plasma from pooled blood samples (n = 5) collected at 30 min and 48 h was performed and subsequently analysed using PROTOMAP