Fig. 5
From: Plasma degradome affected by variable storage of human blood
Prolonged blood storage affects TSP-1 protein levels and degradation. a Gradual increase of TSP-1 protein levels as indicated by label-free quantitative mass spectrometry analysis of tryptic peptides, indicating a 1.3-fold change after 48 h of blood storage (p < 0.001). b TSP-1 protein degradation patterns as observed by the PROTOMAP peptograph (red bars—30 min; blue bars—48 h) and confirmed by western blot analysis at the indicated times. The stars indicated in the PROTOMAP peptograph correspond to the bands in the 120–150 kDa region observed in the western blot, suggesting an increase in protein levels as well as partial degradation