Fig. 6
From: Plasma degradome affected by variable storage of human blood
Minimal proteolysis of complement C4B upon blood storage. Blood sample pools centrifuged after 30 min and 48 h of storage at ambient temperature were analysed by PROTOMAP (red bars—30 min; blue bars—48 h) and anti-complement C4B western blotting. The 40 kDa fragment highlighted with a star (*) was increased as shown by the PROTOMAP peptograph, corresponding to a degradation intermediate also detected by western blotting. This fragment corresponds to the C4d component. Two more fragments of lower MW were increased in the PROTOMAP peptograph, corresponding to ~18 and ~30 kDa