Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

Table 2 Top ten proteins of highest scores detected by LC–MALDI–TOF/TOF

Accession	Description	Score (%)	Matched peptides	Sequence coverage (%)
Top ten proteins identified through LC–MALDI TOF/TOF
EHI_160940	Aldehyde-alcohol dehydrogenase 2, putative	28.67	17	31.8
EHI_150490	Aldehyde-alcohol dehydrogenase 2	28.67	17	31.8
EHI_130700	Enolase, putative	25.52	13	53.2
EHI_009530	Pyruvate phosphate dikinase	20.16	8	30.9
EHI_044970	Malic enzyme, putative	11.96	5	30.4
EHI_178960	Acetyl-CoA synthetase, putative	10.34	5	20.6
EHI_125950	Alcohol dehydrogenase, putative	10	5	23.2
EHI_050330	Malic enzyme, putative	9.84	4	26.7
EHI_051060	Pyruvate: ferredoxin oxidoreductase	8.67	3	16.5
EHI_107290	Actin, putative	8.44	5	8.44

Score (%) Total (ProtScore), a measure of the total amount of evidence for a detected protein. The Total ProtScore is calculated using all the peptides detected for the protein
Matched peptides The number of distinct peptides having at least 95% confidence
Sequence coverage The percentage of matching amino acids from identified peptides having confidence greater than 0 divided by the total number of amino acids in the sequence

ISSN: 1559-0275