Fig. 3From: Development of a microarray-based method for allergen-specific IgE and IgG4 detectionFluorescent signals obtained using different developing systems for two different samples (a and b). The fluorescence of Cy5 for sIgE (green columns: developed with anti-IgE-Cy5 individually; red columns: developed with an anti-IgE-Cy5/anti-IgG4-Cy3 mixture) was registered at 716 ± 22 nm, and the fluorescence of Cy3 for sIgG4 (yellow columns: developed with an anti-IgE-Cy5/anti-IgG4-Cy3 mixture; blue columns: developed with anti-IgG4-Cy3 individually) was registered at 607 ± 35 nmBack to article page